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Issue 1, 2015
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(−)-Epicatechin prevents alterations in the metabolism of superoxide anion and nitric oxide in the hearts of l-NAME-treated rats

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Abstract

The aim of this work was to evaluate the effects of (−)-epicatechin administration in the heart of a rat model with reduced NO production that follows a short-term treatment with L-NAME. Sprague-Dawley rats were treated for 4 d with L-NAME in the absence or presence of (−)-epicatechin in the diet. The redox status in cardiac tissue was improved by (−)-epicatechin administration. L-NAME treatment induced a decrease in NO synthase activity (−62%, p < 0.05) and an increase in NADPH-dependent superoxide anion production (+300%, p < 0.05) that were totally prevented by (−)-epicatechin administration. These effects of (−)-epicatechin were associated with a higher endothelial NO synthase phosphorylation at an activation site and a reduced expression of the regulatory subunit, p47phox, suggesting the involvement of posttranslational mechanisms in (−)-epicatechin action. Thus, the (−)-epicatechin treatment would restore NO steady state levels in vivo through effects on both, its synthesis and degradation via the reaction with superoxide anion. The fact that (−)-epicatechin is commonly present in human diet makes this compound a reasonable explanation for the positive cardiovascular effects of a high consumption of fruits and vegetables.

Graphical abstract: (−)-Epicatechin prevents alterations in the metabolism of superoxide anion and nitric oxide in the hearts of l-NAME-treated rats

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Article information


Submitted
23 Jun 2014
Accepted
04 Oct 2014
First published
31 Oct 2014

Food Funct., 2015,6, 154-160
Article type
Paper

(−)-Epicatechin prevents alterations in the metabolism of superoxide anion and nitric oxide in the hearts of L-NAME-treated rats

B. Piotrkowski, V. Calabró, M. Galleano and C. G. Fraga, Food Funct., 2015, 6, 154
DOI: 10.1039/C4FO00554F

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