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Issue 8, 2016
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The use of the Rx spin label in orientation measurement on proteins, by EPR

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Abstract

The bipedal spin label Rx is more restricted in its conformation and dynamics than its monopodal counterpart R1. To systematically investigate the utility of the Rx label, we have attempted to comprehensively survey the attachment of Rx to protein secondary structures. We have examined the formation, structure and dynamics of the spin label in relation to the underlying protein in order to determine feasibility and optimum conditions for distance and orientation measurement by pulsed EPR. The labeled proteins have been studied using molecular dynamics, CW EPR, pulsed EPR distance measurement at X-band and orientation measurement at W-band. The utility of different modes and positions of attachment have been compared and contrasted.

Graphical abstract: The use of the Rx spin label in orientation measurement on proteins, by EPR

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Publication details

The article was received on 10 Aug 2015, accepted on 25 Sep 2015 and first published on 25 Sep 2015


Article type: Paper
DOI: 10.1039/C5CP04753F
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Citation: Phys. Chem. Chem. Phys., 2016,18, 5799-5806
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    The use of the Rx spin label in orientation measurement on proteins, by EPR

    M. A. Stevens, J. E. McKay, J. L. S. Robinson, H. EL Mkami, G. M. Smith and D. G. Norman, Phys. Chem. Chem. Phys., 2016, 18, 5799
    DOI: 10.1039/C5CP04753F

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