Jump to main content
Jump to site search

Issue 37, 2015
Previous Article Next Article

Direct electron transfer of Phanerochaete chrysosporium cellobiose dehydrogenase at platinum and palladium nanoparticles decorated carbon nanotubes modified electrodes

Author affiliations

Abstract

In the present work, platinum and palladium nanoparticles (PtNPs and PdNPs) were decorated on the surface of multi-walled carbon nanotubes (MWCNTs) by a simple thermal decomposition method. The prepared nanohybrids, PtNPs–MWCNTs and PdNPs–MWCNTs, were cast on the surface of spectrographic graphite electrodes and then Phanerochaete chrysosporium cellobiose dehydrogenase (PcCDH) was adsorbed on the modified layer. Direct electron transfer between PcCDH and the nanostructured modified electrodes was studied using flow injection amperometry and cyclic voltammetry. The maximum current responses (Imax) and the apparent Michaelis–Menten constants (KappM) for the different PcCDH modified electrodes were calculated by fitting the data to the Michaelis–Menten equation and compared. The sensitivity towards lactose was 3.07 and 3.28 μA mM−1 at the PcCDH/PtNPs–MWCNTs/SPGE and PcCDH/PdNPs–MWCNTs/SPGE electrodes, respectively, which were higher than those measured at the PcCDH/MWCNTs/SPGE (2.60 μA mM−1) and PcCDH/SPGE (0.92 μA mM−1). The modified electrodes were additionally tested as bioanodes for biofuel cell applications.

Graphical abstract: Direct electron transfer of Phanerochaete chrysosporium cellobiose dehydrogenase at platinum and palladium nanoparticles decorated carbon nanotubes modified electrodes

Back to tab navigation

Supplementary files

Publication details

The article was received on 01 Jul 2015, accepted on 21 Aug 2015 and first published on 24 Aug 2015


Article type: Paper
DOI: 10.1039/C5CP03812J
Author version
available:
Download author version (PDF)
Citation: Phys. Chem. Chem. Phys., 2015,17, 24157-24165
  •   Request permissions

    Direct electron transfer of Phanerochaete chrysosporium cellobiose dehydrogenase at platinum and palladium nanoparticles decorated carbon nanotubes modified electrodes

    S. Bozorgzadeh, H. Hamidi, R. Ortiz, R. Ludwig and L. Gorton, Phys. Chem. Chem. Phys., 2015, 17, 24157
    DOI: 10.1039/C5CP03812J

Search articles by author

Spotlight

Advertisements