Distance measurements between manganese(II) and nitroxide spin-labels by DEER determine a binding site of Mn2+ in the HP92 loop of ribosomal RNA

Ilia Kaminker; Morgan Bye; Natanel Mendelman; Kristmann Gislason; Snorri Th. Sigurdsson; Daniella Goldfarb

doi:10.1039/C5CP01624J

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Distance measurements between manganese(ii) and nitroxide spin-labels by DEER determine a binding site of Mn²⁺ in the HP92 loop of ribosomal RNA†

Ilia Kaminker,^a Morgan Bye,^a Natanel Mendelman,^a Kristmann Gislason,^a Snorri Th. Sigurdsson^b and Daniella Goldfarb*^b

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* Corresponding authors

^a Department of Chemical Physics, Weizmann Institute of Science, Rehovot 76100, Israel

^b University of Iceland, Department of Chemistry, Science Institute Dunhaga 3, 107 Reykjavik, Iceland

Abstract

Mn²⁺ localization in hairpin 92 of the 23S ribosomal RNA (HP92) was obtained using W-band (95 GHz) DEER (double electron–electron resonance) distance measurements between the Mn²⁺ ion and nitroxide spin labels on the RNA. It was found to be preferably situated in the minor groove of the double strand region close to the HP92 loop.

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Article information

DOI: https://doi.org/10.1039/C5CP01624J
Article type: Communication
Submitted: 20 Mar 2015
Accepted: 14 May 2015
First published: 15 May 2015

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Phys. Chem. Chem. Phys., 2015,17, 15098-15102

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Distance measurements between manganese(II) and nitroxide spin-labels by DEER determine a binding site of Mn²⁺ in the HP92 loop of ribosomal RNA

I. Kaminker, M. Bye, N. Mendelman, K. Gislason, S. Th. Sigurdsson and D. Goldfarb, Phys. Chem. Chem. Phys., 2015, 17, 15098 DOI: 10.1039/C5CP01624J

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