Jump to main content
Jump to site search

Issue 16, 2015
Previous Article Next Article

The role of tryptophans in the UV-B absorption of a UVR8 photoreceptor – a computational study

Author affiliations

Abstract

Arabidopsis thaliana UV RESISTANCE LOCUS8 (UVR8) has been identified as a photoreceptor for ultraviolet-B (UV-B). Tryptophan (Trp) residues have been shown to play a critical role in the response to UV-B irradiation in UVR8. In this work, we explore the spectroscopic behaviors of Trps in different protein environments of the UVR8 structure using the time-dependent density functional tight-binding (TD-DFTB) scheme. We show that W233 exhibits the longest absorption wavelength, highlighting its potential as a terminal Trp chromophore in the UV-B harvesting antenna. Our electronic and optical property analyses using various amino acid models support the important roles of W285 and W233 in sensing UV-B light at longer absorption wavelengths (∼290 nm). We also provide evidence for the specific function of W94 in absorption at the longest wavelengths (305.8 nm in cluster II and 304.5 nm in cluster III). To these findings, we also add information about the influence of the arginine and aspartic acid residues surrounding the Trp pyramid on the particular absorption bands (280–300 nm) that are characteristic of the UV-B photoreceptor.

Graphical abstract: The role of tryptophans in the UV-B absorption of a UVR8 photoreceptor – a computational study

Back to tab navigation

Supplementary files

Publication details

The article was received on 26 Dec 2014, accepted on 18 Mar 2015 and first published on 19 Mar 2015


Article type: Paper
DOI: 10.1039/C4CP06073C
Author version
available:
Download author version (PDF)
Citation: Phys. Chem. Chem. Phys., 2015,17, 10786-10794
  •   Request permissions

    The role of tryptophans in the UV-B absorption of a UVR8 photoreceptor – a computational study

    Q. Wu, B. Huang, T. A. Niehaus, X. Yang, J. Fan and R. Zhang, Phys. Chem. Chem. Phys., 2015, 17, 10786
    DOI: 10.1039/C4CP06073C

Search articles by author

Spotlight

Advertisements