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Issue 69, 2015
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DNA-mediated supercharged fluorescent protein/graphene oxide interaction for label-free fluorescence assay of base excision repair enzyme activity

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Abstract

The interaction between supercharged green fluorescent protein (ScGFP) and graphene oxide (GO) as well as the resulting quenching effect of GO on ScGFP were investigated. Based on this unique quenching effect and the DNA-mediated ScGFP/GO interaction, a label-free fluorescence method has been established for homogeneously assaying the activity and inhibition of base excision repair enzyme.

Graphical abstract: DNA-mediated supercharged fluorescent protein/graphene oxide interaction for label-free fluorescence assay of base excision repair enzyme activity

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The article was received on 09 Jun 2015, accepted on 14 Jul 2015 and first published on 14 Jul 2015


Article type: Communication
DOI: 10.1039/C5CC04759E
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Citation: Chem. Commun., 2015,51, 13373-13376

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    DNA-mediated supercharged fluorescent protein/graphene oxide interaction for label-free fluorescence assay of base excision repair enzyme activity

    Z. Wang, Y. Li, L. Li, D. Li, Y. Huang, Z. Nie and S. Yao, Chem. Commun., 2015, 51, 13373
    DOI: 10.1039/C5CC04759E

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