Issue 13, 2015
From the journal:

Chemical Communications

A high-throughput screening method for determining the substrate scope of nitrilases†

Gary W. Black,a   Nicola L. Brown,a   Justin J. B. Perry,*a   P. David Randall,b   Graeme Turnbulla  and  Meng Zhanga  

* Corresponding authors

a Industrial Biotechnology Research Group, Department of Applied Sciences, Faculty of Health and Life Sciences, Northumbria University, Newcastle upon Tyne, NE1 8ST, UK
E-mail: justin.perry@northumbria.ac.uk

b Chemoxy International Ltd, Middlesbrough, Cleveland, TS3 6AF, UK

Abstract

Nitrile compounds are intermediates in the synthesis of pharmaceuticals such as atorvastatin. We have developed a chromogenic reagent to screen for nitrilase activity as an alternative to Nessler's reagent. It produces a semi-quantifiable blue colour and hydrolysis of 38 nitrile substrates by 23 nitrilases as cell-free extracts has been shown.

Graphical abstract: A high-throughput screening method for determining the substrate scope of nitrilases

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Article information

DOI
https://doi.org/10.1039/C4CC06021K
Article type
Communication
Submitted
01 Aug 2014
Accepted
19 Dec 2014
First published
22 Dec 2014
This article is Open Access
Creative Commons BY license

Chem. Commun., 2015,51, 2660-2662

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A high-throughput screening method for determining the substrate scope of nitrilases

G. W. Black, N. L. Brown, J. J. B. Perry, P. D. Randall, G. Turnbull and M. Zhang, Chem. Commun., 2015, 51, 2660 DOI: 10.1039/C4CC06021K

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