Issue 77, 2015

Rational design of paraoxonase 1 (PON1) for the efficient hydrolysis of organophosphates

Abstract

A rational design of paraoxonase 1 based on molecular docking discovered H115W/T332S and I74F/H115W/T332S mutants exhibited a 40-fold increase in catalytic efficiency (kcat/Km) toward the hydrolysis of two toxic and popular organophosphates (diethyl-paraoxon and dimethyl-paraoxon). Moreover, the conversion of the paraoxons (741.3–825.6 mg L−1) by the evolved mutants was 42–60-fold faster than that by the wild type.

Graphical abstract: Rational design of paraoxonase 1 (PON1) for the efficient hydrolysis of organophosphates

Supplementary files

Article information

Article type
Communication
Submitted
15 Jul 2015
Accepted
12 Aug 2015
First published
12 Aug 2015

Chem. Commun., 2015,51, 14536-14539

Author version available

Rational design of paraoxonase 1 (PON1) for the efficient hydrolysis of organophosphates

Q. A. T. Le, R. Chang and Y. H. Kim, Chem. Commun., 2015, 51, 14536 DOI: 10.1039/C5CC05857K

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