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Issue 57, 2015
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A doubly responsive probe for the detection of Cys4-tagged proteins

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Abstract

Recombinant proteins bearing a tag are crucial tools for assessing protein location or function. Small tags such as Cys4 tag (tetracysteine; Cys–Cys–X–X–Cys–Cys) are less likely disrupt protein function in the living cell than green fluorescent protein. Herein we report the first example of the design and synthesis of a dual fluorescence and hyperpolarized 129Xe NMR-based sensor of Cys4-tagged proteins. This sensor becomes fluorescent when bound to such Cys4-tagged peptides, and the 129Xe NMR spectrum exhibits a specific signal, characteristic of the biosensor-peptide association.

Graphical abstract: A doubly responsive probe for the detection of Cys4-tagged proteins

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Supplementary files

Article information


Submitted
08 Jun 2015
Accepted
12 Jun 2015
First published
12 Jun 2015

Chem. Commun., 2015,51, 11482-11484
Article type
Communication
Author version available

A doubly responsive probe for the detection of Cys4-tagged proteins

N. Kotera, E. Dubost, G. Milanole, E. Doris, E. Gravel, N. Arhel, T. Brotin, J.-P. Dutasta, J. Cochrane, E. Mari, C. Boutin, E. Léonce, P. Berthault and B. Rousseau, Chem. Commun., 2015, 51, 11482
DOI: 10.1039/C5CC04721H

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