We report the use of a molecular peroxidase mimic biuret-Fe-TAML for chemoselective labeling of proteins and the subsequent visual detection (<0.1 pmoles) of the conjugate in a polyacrylamide gel by catalytic signal amplification. Use of this probe in activity based protein profiling (ABPP) of serine proteases is also demonstrated.
S. Kumari, C. Panda, S. Mazumdar and S. Sen Gupta, Chem. Commun., 2015, 51, 15257 DOI: 10.1039/C5CC04399A
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