Issue 46, 2015

The determination of DNA methyltransferase activity by quenching of tris(2,2′-bipyridine)ruthenium electrogenerated chemiluminescence with ferrocene

Abstract

An electrogenerated chemiluminescence (ECL) biosensing method for the determination of DNA methyltransferase activity is developed by the quenching of tris(2,2′-bipyridine)ruthenium ECL by ferrocene, and it is demonstrated that the ECL biosensing method measures DNA adenine methylation methyltransferase over a dynamic concentration range (0.1 U mL−1–100 U mL−1) with an extremely low detection limit of 0.03 U mL−1, using gold nanoparticles and a quenching ECL signal produced by a chemical quencher such as ferrocene.

Graphical abstract: The determination of DNA methyltransferase activity by quenching of tris(2,2′-bipyridine)ruthenium electrogenerated chemiluminescence with ferrocene

Supplementary files

Article information

Article type
Communication
Submitted
04 Apr 2015
Accepted
05 May 2015
First published
06 May 2015

Chem. Commun., 2015,51, 9487-9490

Author version available

The determination of DNA methyltransferase activity by quenching of tris(2,2′-bipyridine)ruthenium electrogenerated chemiluminescence with ferrocene

X. Luo, Y. Li, J. Zheng, H. Qi, Z. Liang and X. Ning, Chem. Commun., 2015, 51, 9487 DOI: 10.1039/C5CC02817E

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