Issue 54, 2015
From the journal:

Chemical Communications

An enhanced ascorbate peroxidase 2/antibody-binding domain fusion protein (APEX2–ABD) as a recombinant target-specific signal amplifier†

Jisu Lee,a   Eun Kyung Song,a   Yoonji Bae,a   Junseon Min,a   Hyun-Woo Rhee,b   Tae Joo Park,a   Moonil Kim*c  and  Sebyung Kang*a  

* Corresponding authors

a Department of Biological Sciences, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan, Korea
E-mail: sabsab7@unist.ac.kr
Fax: +82-52-217-5309
Tel: +82-52-217-5325

b Department of Chemistry, School of Natural Science, Ulsan National Institute of Science and Technology (UNIST), Ulsan, Korea

c BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea
E-mail: kimm@kribb.re.kr
Fax: +82-42-879-8594
Tel: +82-42-879-8447

Abstract

A recombinant target-specific signal amplifier was constructed by genetically fusing the enhanced ascorbate peroxidase 2 (APEX2) and an antibody-binding domain (ABD). The fusion protein APEX2–ABD possessed the peroxidase activity and the antibody-binding capability simultaneously and replaced the conventional HRP-conjugated secondary antibodies in a TSA assay for amplifying fluorescence signals.

Graphical abstract: An enhanced ascorbate peroxidase 2/antibody-binding domain fusion protein (APEX2–ABD) as a recombinant target-specific signal amplifier

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Article information

DOI
https://doi.org/10.1039/C5CC02409A
Article type
Communication
Submitted
23 Mar 2015
Accepted
27 May 2015
First published
11 Jun 2015

Chem. Commun., 2015,51, 10945-10948

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An enhanced ascorbate peroxidase 2/antibody-binding domain fusion protein (APEX2–ABD) as a recombinant target-specific signal amplifier

J. Lee, E. K. Song, Y. Bae, J. Min, H. Rhee, T. J. Park, M. Kim and S. Kang, Chem. Commun., 2015, 51, 10945 DOI: 10.1039/C5CC02409A

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