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Issue 52, 2015
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Molecular basis of Streptococcus mutans sortase A inhibition by the flavonoid natural product trans-chalcone

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Abstract

Sortase A (SrtA) from Gram positive pathogens is an attractive target for inhibitors due to its role in the attachment of surface proteins to the cell wall. We found that the plant natural product trans-chalcone inhibits Streptococcus mutans SrtA in vitro and also inhibited S. mutans biofilm formation. Mass spectrometry revealed that the trans-chalcone forms a Michael addition adduct with the active site cysteine. The X-ray crystal structure of the SrtA H139A mutant provided new insights into substrate recognition by the sortase family. Our study suggests that chalcone flavonoids have potential as sortase-specific oral biofilm inhibitors.

Graphical abstract: Molecular basis of Streptococcus mutans sortase A inhibition by the flavonoid natural product trans-chalcone

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Publication details

The article was received on 03 Mar 2015, accepted on 12 May 2015 and first published on 18 May 2015


Article type: Communication
DOI: 10.1039/C5CC01816A
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Citation: Chem. Commun., 2015,51, 10483-10485
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    Molecular basis of Streptococcus mutans sortase A inhibition by the flavonoid natural product trans-chalcone

    D. J. Wallock-Richards, J. Marles-Wright, D. J. Clarke, A. Maitra, M. Dodds, B. Hanley and D. J. Campopiano, Chem. Commun., 2015, 51, 10483
    DOI: 10.1039/C5CC01816A

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