Issue 34, 2015

4-Phosphopyrazol-2-yl alanine: a non-hydrolysable analogue of phosphohistidine

Abstract

We report the synthesis of a stable analogue of τ-phosphohistidine: 4-phosphopyrazol-2-yl alanine (pPza). Polyclonal antibodies generated against the mimic show high reactivity and selectivity for τ-phosphohistidine, with minor or no cross-reactivity towards non-phosphorylated histidine or O-phosphoamino acids, including phosphotyrosine.

Graphical abstract: 4-Phosphopyrazol-2-yl alanine: a non-hydrolysable analogue of phosphohistidine

Supplementary files

Article information

Article type
Communication
Submitted
03 Mar 2015
Accepted
18 Mar 2015
First published
25 Mar 2015
This article is Open Access
Creative Commons BY license

Chem. Commun., 2015,51, 7305-7308

Author version available

4-Phosphopyrazol-2-yl alanine: a non-hydrolysable analogue of phosphohistidine

M. Lilley, B. Mambwe, M. J. Thompson, R. F. W. Jackson and R. Muimo, Chem. Commun., 2015, 51, 7305 DOI: 10.1039/C5CC01811K

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