Elucidating the relationship between superoxide anion levels and lifespan using an enhanced two-photon fluorescence imaging probe†
Abstract
We report a new reversible fluorescent two-photon (TP) probe (PY-CA) with high TP absorption cross section and pH-independent fluorescence response, which allow monitoring of O2˙− fluxes dynamically, selectively and sensitively. The imaging results indicate that O2˙− at high levels can shorten the life of Caenorhabditis elegans.