Issue 27, 2015
From the journal:

Chemical Communications

Phosphorylation-induced hybridization chain reaction on beads: an ultrasensitive flow cytometric assay for the detection of T4 polynucleotide kinase activity†

Yuecheng Zhang,a   Chenghui Liu,*a   Sujuan Sun,a   Yanli Tanga  and  Zhengping Li*a  

* Corresponding authors

a Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, Shaanxi Province, P. R. China
E-mail: liuch@snnu.edu.cn, lzpbd@snnu.edu.cn
Fax: +86 29 81530859
Tel: +86 29 81530859

Abstract

A versatile flow cytometric bead assay (FCBA) has been developed for the ultrasensitive detection of T4 PNK activity by integrating the advantages of the phosphorylation-induced hybridization chain reaction (HCR) for fluorescence signal amplification and flow cytometry for the robust, sensitive and rapid signal readout of the microbeads (MBs).

Graphical abstract: Phosphorylation-induced hybridization chain reaction on beads: an ultrasensitive flow cytometric assay for the detection of T4 polynucleotide kinase activity

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C5CC00572H
Article type
Communication
Submitted
21 Jan 2015
Accepted
05 Feb 2015
First published
05 Feb 2015

Chem. Commun., 2015,51, 5832-5835

Permissions

Request permissions

Phosphorylation-induced hybridization chain reaction on beads: an ultrasensitive flow cytometric assay for the detection of T4 polynucleotide kinase activity

Y. Zhang, C. Liu, S. Sun, Y. Tang and Z. Li, Chem. Commun., 2015, 51, 5832 DOI: 10.1039/C5CC00572H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements