Issue 10, 2015

A sensitive detection of T4 polynucleotide kinase activity based on β-cyclodextrin polymer enhanced fluorescence combined with an exonuclease reaction

Abstract

A strategy for T4 polynucleotide kinase activity detection was proposed based on a β-cyclodextrin polymer (polyβ-CD) and an exonuclease reaction. The fluorescence of pyrene enhanced by more than 10 times in the presence of polyβ-CD, and a simple detection of T4 PNK was achieved with a detection limit of 0.02 units per mL.

Graphical abstract: A sensitive detection of T4 polynucleotide kinase activity based on β-cyclodextrin polymer enhanced fluorescence combined with an exonuclease reaction

Supplementary files

Article information

Article type
Communication
Submitted
11 Nov 2014
Accepted
10 Dec 2014
First published
10 Dec 2014

Chem. Commun., 2015,51, 1815-1818

Author version available

A sensitive detection of T4 polynucleotide kinase activity based on β-cyclodextrin polymer enhanced fluorescence combined with an exonuclease reaction

C. Song, X. Yang, K. Wang, Q. Wang, J. Liu, J. Huang, L. He, P. Liu, Z. Qing and W. Liu, Chem. Commun., 2015, 51, 1815 DOI: 10.1039/C4CC08991J

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