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Issue 17, 2015
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Rationally engineered variants of S-adenosylmethionine (SAM) synthase: reduced product inhibition and synthesis of artificial cofactor homologues

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Abstract

S-Adenosylmethionine (SAM) synthase was engineered for biocatalytic production of SAM and long-chain analogues by rational re-design. Substitution of two conserved isoleucine residues extended the substrate spectrum of the enzyme to artificial S-alkylhomocysteines. The variants proved to be beneficial in preparative synthesis of SAM (and analogues) due to a much reduced product inhibition.

Graphical abstract: Rationally engineered variants of S-adenosylmethionine (SAM) synthase: reduced product inhibition and synthesis of artificial cofactor homologues

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Publication details

The article was received on 27 Oct 2014, accepted on 02 Jan 2015 and first published on 02 Jan 2015


Article type: Communication
DOI: 10.1039/C4CC08478K
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Chem. Commun., 2015,51, 3637-3640

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    Rationally engineered variants of S-adenosylmethionine (SAM) synthase: reduced product inhibition and synthesis of artificial cofactor homologues

    M. Dippe, W. Brandt, H. Rost, A. Porzel, J. Schmidt and L. A. Wessjohann, Chem. Commun., 2015, 51, 3637
    DOI: 10.1039/C4CC08478K

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