Jump to main content
Jump to site search

Issue 20, 2015
Previous Article Next Article

A fully automated analyzer for multiple detection of allergen-specific immunoglobulin E

Author affiliations

Abstract

Allergy testing is important for evidence-based care and treatment. In this paper, we report a new clinical laboratory instrument, AdvanSure AlloStation Smart, which is used for a multiple allergen-specific immunoglobulin (IgE) assay. For efficient automation of immunoblot procedures, a novel tilting carousel technology was incorporated. The analyzer showed 100.5–101.7% of pipetting accuracy, no sample carryover, and 0.2–3.2% coefficient of variation of optical precision. Assay performance on the system was comparable with that of a single allergen test, ImmunoCAP for 23 allergens. The slope and the intercept of this comparison were 0.994 and 0.070, respectively. The Pearson correlation coefficient between two methods was r = 0.836. The coefficient of variation of intra-assay precision ranged from 3.2% to 11.1% and that of inter-assay precision varied from 4.0% to 17.6%. The AdvanSure AlloStation Smart provides a simple and reliable multiple assay platform to monitor allergen-specific IgE profiles.

Graphical abstract: A fully automated analyzer for multiple detection of allergen-specific immunoglobulin E

Back to tab navigation

Publication details

The article was received on 25 Jun 2015, accepted on 02 Sep 2015 and first published on 04 Sep 2015


Article type: Paper
DOI: 10.1039/C5AY01634G
Citation: Anal. Methods, 2015,7, 8889-8895
  •   Request permissions

    A fully automated analyzer for multiple detection of allergen-specific immunoglobulin E

    J. H. Oh, M. K. Park, S. W. Kim and J. Park, Anal. Methods, 2015, 7, 8889
    DOI: 10.1039/C5AY01634G

Search articles by author

Spotlight

Advertisements