A HFCF-UF method to directly analyze the total concentration of protein-binding drugs

Abstract

In the present study, a direct injection hollow fiber centrifugal ultrafiltration (HFCF-UF) method was developed for therapeutic drug monitoring (TDM) for the total concentration of liposoluble protein binding drugs. We used carbamazepine (CBZ) and phenobarbital (PB) as model drugs and proposed a HFCF-UF method to analyze the total concentration of liposoluble protein binding drugs without the use of traditional biological sample preparations. Our results demonstrate that acetone acts as a liposoluble desorption agent and can make the target compounds release from the protein–drug complexes and then re-dissolve the released compounds. The absolute recovery is excellent at approximately 100%, which is higher than when traditional biological sample preparations have to be used. Calibration curves can be established in a plasma-free standard solution instead of using spiked human plasma. The HFCF-UF method offers the advantages of highly satisfactory performance and simple and fast pretreatment, consistent with the practical requirements of TDM.