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Issue 19, 2015
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Simultaneous determination of 41 components in Gualou Guizhi granules by UPLC coupled with triple quadrupole mass spectrometry

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Abstract

Gualou Guizhi granules (GLGZGs) are a classical formula of traditional Chinese medicine, which have been commonly used to treat dysfunction after stroke, epilepsy and spinal cord injury. In this study, an efficient method using ultra-performance liquid chromatography (UPLC) coupled with triple quadrupole mass spectrometry (QqQ MS) was developed for the rapid determination of forty-one major components in GLGZGs. Chromatographic separation was achieved on a Waters ACQUITY UPLC Cortest C18 column (100 mm × 2.1 mm, 1.6 μm) with a gradient mobile phase (A: 0.1% aqueous formic acid and B: acetonitrile with 0.1% formic acid) at a flow rate of 0.25 mL min−1. Multiple-reaction monitoring (MRM) was used for quantitative analysis. Ten batches of GLGZGs were analyzed with a good linear regression relationship (R2, 0.9841–0.9998). The present study offered highly sensitive, specific and speedy determination of forty-one components, which promoted the quality control investigation of GLGZGs via employing the developed method.

Graphical abstract: Simultaneous determination of 41 components in Gualou Guizhi granules by UPLC coupled with triple quadrupole mass spectrometry

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Publication details

The article was received on 22 May 2015, accepted on 10 Aug 2015 and first published on 13 Aug 2015


Article type: Paper
DOI: 10.1039/C5AY01336D
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Anal. Methods, 2015,7, 8285-8296

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    Simultaneous determination of 41 components in Gualou Guizhi granules by UPLC coupled with triple quadrupole mass spectrometry

    Y. Lin, W. Xu, W. Xu, M. Huang, Y. Zhang, H. Li, K. Chu and L. Chen, Anal. Methods, 2015, 7, 8285
    DOI: 10.1039/C5AY01336D

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