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Issue 16, 2015
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Development of a 15-class multiresidue method for analyzing 78 hydrophilic and hydrophobic veterinary drugs in milk, egg and meat by liquid chromatography-tandem mass spectrometry

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Abstract

The development of a multiclass, multiresidue method for analyzing veterinary drugs in food is the recent trend of research for regulatory monitoring laboratories. This work reports a multiclass method for the determination of both hydrophilic and hydrophobic veterinary drug residues, including anthelmintics, avermectins, benzimidazoles, β-agonists, β-lactams, coccidiostats, corticosteroids/steroids, lincosamides, macrolides, non-steroidal anti-inflammatory drugs, phenicols, quinolones, tranquillisers, antiviral drugs and some other veterinary drugs, in milk, egg and meat. By using liquid–liquid extraction low temperature partition with centrifugation for acetonitrile (MeCN)/aqueous phase separation, hydrophilic and hydrophobic drugs were recovered separately. The MeCN phase was cleaned up with dispersive solid phase extraction. To achieve retention and separation of veterinary drugs with a wide range of polarity, ultra-performance hydrophilic interaction liquid chromatography (HILIC) and reversed phase liquid chromatography (RPLC) coupled to a tandem mass spectrometer (MS/MS) were employed. The method was successfully validated. The recoveries of the method in general ranged from 70 to 120% with precision RSD ≤20%. The limits of quantification of the method ranged from 0.1 to 10 μg kg−1 for the targeted veterinary drugs.

Graphical abstract: Development of a 15-class multiresidue method for analyzing 78 hydrophilic and hydrophobic veterinary drugs in milk, egg and meat by liquid chromatography-tandem mass spectrometry

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Article information


Submitted
21 May 2015
Accepted
09 Jul 2015
First published
10 Jul 2015

Anal. Methods, 2015,7, 6764-6776
Article type
Paper
Author version available

Development of a 15-class multiresidue method for analyzing 78 hydrophilic and hydrophobic veterinary drugs in milk, egg and meat by liquid chromatography-tandem mass spectrometry

S. W. C. Chung and C. Lam, Anal. Methods, 2015, 7, 6764
DOI: 10.1039/C5AY01317H

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