Development of sandwich ELISA and immunochromatographic strip methods for the detection of Xanthomonas oryzae pv. oryzae

Abstract

A pair of sensitive monoclonal antibodies against both Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola were generated by immunizing mice and cell fusion techniques. The sandwich enzyme-linked immunosorbent assay method and immunochromatographic test strip were developed based on newly generated monoclonal antibodies for detection in rice samples. The lower limit of detection of the sandwich ELISA method for X. oryzae pv. oryzae was 1.0 × 10⁴ cfu mL⁻¹. The cut-off values for the immunochromatographic test strip for X. oryzae pv. oryzae and X. oryzae pv. oryzicola in rice samples were 3.3 × 10⁵ cfu mL⁻¹ and 1 × 10⁶ cfu mL⁻¹, respectively. The quantitative sandwich ELISA method and rapid immunochromatographic test strip assay could be useful for food safety inspections.