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Issue 21, 2015
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Benzimidazole and imidazole fungicide analysis in grape and wine samples using a competitive enzyme-linked immunosorbent assay

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Abstract

This manuscript reports a quantitative pesticide residue method analysis employing an enzyme-linked immunosorbent assay (ELISA) for detecting carbendazim, imazalil and thiabendazole pesticide residues in vegetable samples. The results have been compared with those obtained from a liquid chromatography system coupled to a triple quadrupole mass spectrometer (LC-ESI-QqQ-MS). A validation study was undertaken to establish this method's performance characteristics on the 3 pesticide residues in two commodities (table grapes and wine). The carbendazim, imazalil and thiabendazole ELISA showed an IC50 reporting limit ranging from 0.5 to 13.8 µg kg−1. The limit of quantification of the developed assay was 5 µg kg−1 for both grape and wine matrices. The optimised assay was compared with a reference procedure based on liquid chromatography using samples acquired from various supermarkets, and a good correlation between both methods was found.

Graphical abstract: Benzimidazole and imidazole fungicide analysis in grape and wine samples using a competitive enzyme-linked immunosorbent assay

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Article information


Submitted
22 Apr 2015
Accepted
15 Sep 2015
First published
17 Sep 2015

Anal. Methods, 2015,7, 9158-9165
Article type
Paper

Benzimidazole and imidazole fungicide analysis in grape and wine samples using a competitive enzyme-linked immunosorbent assay

A. Uclés, A. V. García, M. D. Gil García, A. M. Aguilera del Real and A. R. Fernández-Alba, Anal. Methods, 2015, 7, 9158
DOI: 10.1039/C5AY01048A

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