Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 11, 2015
Previous Article Next Article

Development of an enzyme-linked immunosorbent assay for chenodeoxycholic acid using an anti-chenodeoxycholic acid monoclonal antibody

Author affiliations

Abstract

CDCA is one of the major bile acids in humans and has important clinical significance and therapeutic applications. In this work, a new monoclonal antibody (MAb) specific for chenodeoxycholic acid (CDCA) was prepared and characterized. A hybridoma secreting an anti-CDCA MAb was produced by fusing splenocytes from a mouse immunized against a CDCA–BSA conjugate with the hypoxanthine–aminopterin–thymidine (HAT)-sensitive mouse myeloma cell line Sp2/0-Ag14. The antibody was highly specific for CDCA, exhibited only weak reactions with CA and DCA and did not react with other structurally related chemicals. Subsequently, a MAb-based enzyme-linked immunosorbent assay (ELISA) against CDCA was developed and characterized. In this assay, we detected an effective CDCA measurement range of 32 ng mL−1 to 1024 ng mL−1 (R2 = 0.9962). Both intra-assay and inter-assay repeatability and precision were achieved with relative standard deviations (RSD) below 10%. Additionally, the CDCA levels in medicines and samples were determined with high sensitivity and efficiency. This study provides a useful method for detecting CDCA in medicines and will contribute to the further clinical research.

Graphical abstract: Development of an enzyme-linked immunosorbent assay for chenodeoxycholic acid using an anti-chenodeoxycholic acid monoclonal antibody

Back to tab navigation

Article information


Submitted
19 Mar 2015
Accepted
23 Apr 2015
First published
24 Apr 2015

Anal. Methods, 2015,7, 4583-4589
Article type
Paper

Development of an enzyme-linked immunosorbent assay for chenodeoxycholic acid using an anti-chenodeoxycholic acid monoclonal antibody

Y. Zhang, H. Qu, H. Feng, X. Wang, W. Shan, W. Zeng, Q. Wang and Y. Zhao, Anal. Methods, 2015, 7, 4583
DOI: 10.1039/C5AY00733J

Social activity

Search articles by author

Spotlight

Advertisements