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Issue 16, 2015
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A sensitive LC-ESI-MS/MS method for the determination of clotrimazole in human plasma

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Abstract

A rapid, sensitive and specific liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method for the determination of clotrimazole in human plasma was developed and validated. The plasma samples were extracted with a mixed solvent of methyl tert-butyl ether–dichloromethane (4 : 1, v/v), and estazolam was selected as the internal standard. Then, the separation was carried out on a Phenomenex Luna CN column (2.0 × 150 mm, 5 μm), using a mobile phase of methanol-0.1% aqueous formic acid solution (85 : 15, v/v). A triple quadrupole mass spectrometer with the positive ionization mode was used for the determination of target analytes. The monitored ion transitions were m/z 276.9 → 165.1 for clotrimazole and m/z 294.9 → 266.9 for estazolam, respectively. The calibration curve of clotrimazole was established over the range of 0.01563–1.000 ng mL−1 (r2 = 0.9935). The intra- and inter-day precisions were less than 10% and all the biases were not more than 9%. The mean extraction recovery of clotrimazole was greater than 68.4% and no significant matrix effect was detected. The LLOQ of 0.01563 ng mL−1 is sensitive enough to perform pharmacokinetic studies after clotrimazole administration.

Graphical abstract: A sensitive LC-ESI-MS/MS method for the determination of clotrimazole in human plasma

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Publication details

The article was received on 20 Mar 2015, accepted on 08 Jul 2015 and first published on 08 Jul 2015


Article type: Paper
DOI: 10.1039/C5AY00729A
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Anal. Methods, 2015,7, 6672-6677

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    A sensitive LC-ESI-MS/MS method for the determination of clotrimazole in human plasma

    R. Zhou, F. Tang, S. Li, X. Xie, J. Peng, F. Xie, L. Mu and P. Yu, Anal. Methods, 2015, 7, 6672
    DOI: 10.1039/C5AY00729A

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