Jump to main content
Jump to site search

Issue 14, 2015
Previous Article Next Article

A simple, highly sensitive, high throughput and organic solvent-free screening method for melamine by microsphere-based flow cytometry immunoassay

Author affiliations

Abstract

In recent years, the safety problem of infant formula powder has provoked panic among Chinese citizens. One of the most notorious incidents was the 2008 Chinese milk scandal which harmed thousands of infants. Therefore the development of a fast, high throughput screening method towards melamine and other harmful chemicals is of paramount importance. Microsphere-based flow cytometry is a new multiplexing immunochemical method for food-testing. Through competition between the free analyte in the sample and analyte-coupled microspheres, the content of the analyte can be indirectly quantified by measuring the fluorescence emitted from labelled antibodies. This developed method of high sensitivity (limit of detection = 0.70 ng ml−1) has already been validated and successfully applied to screen for melamine in various brands of infant formula powder analysis fulfilling the legislated tolerance level in a simple, fast, high throughput and organic solvent-free manner.

Graphical abstract: A simple, highly sensitive, high throughput and organic solvent-free screening method for melamine by microsphere-based flow cytometry immunoassay

Back to tab navigation

Supplementary files

Publication details

The article was received on 12 Mar 2015, accepted on 06 Jun 2015 and first published on 09 Jun 2015


Article type: Paper
DOI: 10.1039/C5AY00648A
Author version
available:
Download author version (PDF)
Anal. Methods, 2015,7, 5989-5995

  •   Request permissions

    A simple, highly sensitive, high throughput and organic solvent-free screening method for melamine by microsphere-based flow cytometry immunoassay

    T. Tsoi and W. Wong, Anal. Methods, 2015, 7, 5989
    DOI: 10.1039/C5AY00648A

Search articles by author

Spotlight

Advertisements