A MEKC method for naringenin from natural and biological samples

Abstract

The work reported describes the development of a micellar electrokinetic chromatographic (MEKC) method for the determination of naringenin in real samples including grapefruit juice and human blood serum using a PDA detector. The effects of different CE parameters such as concentration and pH of the running buffer, voltage, injection time and concentration of sodium dodecyl sulfate (SDS) were optimized. Under the optimized conditions, naringenin could be well determined within 6 min using 40 mM borate buffer, 40 mM SDS at pH 9.0, at an applied voltage of 25 kV. For the quantitative determination of naringenin (flavonoid aglycone) in grapefruit juice, the naringin (flavonoid glycoside) was hydrolysed and the resulting aglycone was identified and quantified. The calibration curve was linear in the studied concentration range of 0.1 to 50 μg mL⁻¹ (R² = 0.995). The detection limit and the limit of quantification were found to be 0.05 and 0.19 μg mL⁻¹, respectively.