Issue 11, 2015

Preparation and application of a novel mixed-mode monolith for reversed-phase and per aqueous capillary electrochromatography

Abstract

A novel mixed-mode monolithic stationary phase based on bonding of 4,5-imidazoledicarboxy acid to the surface of an organic-silica hybrid monolith was prepared and characterized. Satisfactory characteristics in terms of mechanical stability, permeability and reproducibility of the resultant monolithic column were achieved. A typical per aqueous chromatographic behavior was observed in water-rich mobile phases. The resultant monolithic column was applied for the efficient resolution of various polar and hydrophilic compounds under per aqueous or reversed-phase chromatographic conditions, which successfully demonstrated its suitability for the analysis of these polar compounds in highly aqueous mobile phases. The highest column efficiency obtained for the amines was 149 000 N m−1. The experimental results showed that the mixed-mode mechanism of hydrophobic and ion-exchange interactions was involved in the separation under the given conditions. The successful applications suggested that the mixed-mode organic-silica hybrid monolithic column could offer a wide range of retention behaviors and flexible selectivities toward polar compounds, achieving various target separations.

Graphical abstract: Preparation and application of a novel mixed-mode monolith for reversed-phase and per aqueous capillary electrochromatography

Supplementary files

Article information

Article type
Paper
Submitted
09 Mar 2015
Accepted
01 May 2015
First published
01 May 2015

Anal. Methods, 2015,7, 4750-4756

Preparation and application of a novel mixed-mode monolith for reversed-phase and per aqueous capillary electrochromatography

S. Tang, Y. Guo, X. Liang, F. Wei, L. Yang, S. Liu, X. Liu and S. Jiang, Anal. Methods, 2015, 7, 4750 DOI: 10.1039/C5AY00611B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements