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Issue 7, 2015
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High precision and selectivity for quantitation of enrofloxacin and ciprofloxacin in five chicken tissues using solid phase extraction and ESI LC-MS/MS for application in monitoring residues

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Abstract

Fluoroquinolones (FQs) are synthetic antimicrobials commonly used in intensive poultry farming to treat chronic respiratory disease, colibacillosis, and fowl cholera. As these drugs are also important in treating infections in humans, their use in poultry has been restricted in some countries to avoid the development of antimicrobial-resistant bacteria. In this work, a novel simple and efficient LC-MS/MS method using solid phase extraction (SPE), ESI ionization and multiple reaction mode (MRM) has been developed and validated to determine the two most common FQ residues: enrofloxacin (ENR) and ciprofloxacin (CIP) in chicken target tissues (muscle, liver, kidney, fat and skin) and in plasma. The method is shown to be sensitive, with limits of quantification (LOQ) between 1 ng g−1 and 5 ng g−1, recoveries 93–115% and correlation coefficients greater than 0.99. This novel methodology allows faster and simpler sample workflow compared to previously reported methodologies, making it ideal for high-throughput screening and confirmation of both ENR and CIP meeting the international regulation levels.

Graphical abstract: High precision and selectivity for quantitation of enrofloxacin and ciprofloxacin in five chicken tissues using solid phase extraction and ESI LC-MS/MS for application in monitoring residues

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Supplementary files

Article information


Submitted
12 Dec 2014
Accepted
25 Feb 2015
First published
02 Mar 2015

Anal. Methods, 2015,7, 3291-3297
Article type
Paper
Author version available

High precision and selectivity for quantitation of enrofloxacin and ciprofloxacin in five chicken tissues using solid phase extraction and ESI LC-MS/MS for application in monitoring residues

S. P. Gobbo Ferrari, K. P. D. Bonassa, M. B. Coelho, C. R. Ferreira, H. Falcão da Costa, J. L. P. Jara, M. C. V. Miguel, F. G. R. Reyes, M. N. Eberlin, G. P. Nogueira and R. C. Simas, Anal. Methods, 2015, 7, 3291
DOI: 10.1039/C4AY02962C

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