Issue 4, 2015

Spectrophotometric determination of ethyl carbamate through bi-enzymatic cascade reactions

Abstract

A highly sensitive spectrophotometric method for ethyl carbamate (EC) determination was established through glutamate dehydrogenase/urethanase cascade reactions and the corresponding change in NADH concentration. The absorbance at 340 nm is linearly related to the EC concentration within the range of 0.3–50 μM, with a low detection limit of 0.00928 μM. The assay was further applied to analyse EC in mimic Chinese rice wine samples.

Graphical abstract: Spectrophotometric determination of ethyl carbamate through bi-enzymatic cascade reactions

Supplementary files

Article information

Article type
Communication
Submitted
12 Nov 2014
Accepted
04 Dec 2014
First published
05 Dec 2014

Anal. Methods, 2015,7, 1261-1264

Spectrophotometric determination of ethyl carbamate through bi-enzymatic cascade reactions

X. Lu, N. Zhou and Y. Tian, Anal. Methods, 2015, 7, 1261 DOI: 10.1039/C4AY02693D

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