Issue 1, 2015

Facile colorimetric detection of human chorionic gonadotropin based on the peptide-induced aggregation of gold nanoparticles

Abstract

The human chorionic gonadotropin (hCG) assay for ectopic pregnancy is typically conducted using an enzyme immunoassay (EIA); however, EIA is complicated and time-consuming. Herein, we developed a simple, rapid and sensitive colorimetric assay for hCG based on the anti-aggregation of unmodified gold nanoparticles (AuNPs). The introduction of positively charged hCG-specific peptides into an AuNP solution causes the aggregation of the AuNPs, resulting in a color change from red to blue. When hCG is present, peptide/hCG complexes are formed, thus inhibiting the peptide-induced AuNP aggregation. The dynamic range for hCG detection ranged from 50 to 1000 mIU mL−1, with a detection limit of 25 mIU mL−1. Additionally, this colorimetric sensor was successfully evaluated with serum samples spiked with hCG, demonstrating its ability to be used for the onsite monitoring of hCG.

Graphical abstract: Facile colorimetric detection of human chorionic gonadotropin based on the peptide-induced aggregation of gold nanoparticles

Supplementary files

Article information

Article type
Communication
Submitted
16 Jun 2014
Accepted
30 Sep 2014
First published
01 Oct 2014

Anal. Methods, 2015,7, 29-33

Facile colorimetric detection of human chorionic gonadotropin based on the peptide-induced aggregation of gold nanoparticles

C. Chang, C. Chen, C. Chen and C. Lin, Anal. Methods, 2015, 7, 29 DOI: 10.1039/C4AY01429D

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