Issue 17, 2015
From the journal:

Analyst

Analysis of ATP and AMP binding to a DNA aptamer and its imidazole-tethered derivatives by surface plasmon resonance†

Jing Zhao,a   Satoshi Katsube,b   Junpei Yamamoto,b   Kazuhiko Yamasaki,a   Makoto Miyagishia  and  Shigenori Iwai*b  

* Corresponding authors

a Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan

b Division of Chemistry, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan
E-mail: iwai@chem.es.osaka-u.ac.jp
Fax: +81-6-6850-6240
Tel: +81-6-6850-6250

Abstract

Imidazole was tethered to the C5 position of thymine in an ATP-binding DNA aptamer with two types of linkers, and the affinities of each aptamer for ATP and AMP were determined by surface plasmon resonance measurements. The imidazole-tethered aptamers exhibited higher affinity for ATP, almost independently of the linker structure or the modification site.

Graphical abstract: Analysis of ATP and AMP binding to a DNA aptamer and its imidazole-tethered derivatives by surface plasmon resonance

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Article information

DOI
https://doi.org/10.1039/C5AN01347J
Article type
Communication
Submitted
05 Jul 2015
Accepted
16 Jul 2015
First published
16 Jul 2015

Analyst, 2015,140, 5881-5884

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Analysis of ATP and AMP binding to a DNA aptamer and its imidazole-tethered derivatives by surface plasmon resonance

J. Zhao, S. Katsube, J. Yamamoto, K. Yamasaki, M. Miyagishi and S. Iwai, Analyst, 2015, 140, 5881 DOI: 10.1039/C5AN01347J

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