Issue 18, 2015
From the journal:

Analyst

Single molecular recognition force spectroscopy study of a DNA aptamer with the target epithelial cell adhesion molecule†

Nan Wang,ab   Huiqing Liu,ab   Jinhui Hao,ab   Xiaojing Bai,ab   Huiyan Li,ac   Zhe Zhang,ab   Hongda Wangab  and  Jilin Tang*a  

* Corresponding authors

a State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, P. R. China
E-mail: jltang@ciac.ac.cn
Fax: +86-0431-85262734
Tel: +86-0431-85262734

b Graduate School of Chinese Academy of Sciences, Beijing, P. R. China

c School of Public Health, Jilin University, 1163 Xinmin Street, Changchun 130021, P.R. China

Abstract

The epithelial cell adhesion molecule (EpCAM) is a tumor-specific antigen for malignancies of the epithelialis lineage. In this study the interaction between the DNA-based EpCAM aptamer (SYL3C) and EpCAM was explored using single molecular recognition force spectroscopy (SMFS). The capability of aptamer SYL3C to recognize the EpCAM protein and the kinetic parameters were investigated.

Graphical abstract: Single molecular recognition force spectroscopy study of a DNA aptamer with the target epithelial cell adhesion molecule

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Supplementary files

Article information

DOI
https://doi.org/10.1039/C5AN00945F
Article type
Communication
Submitted
11 May 2015
Accepted
21 Jul 2015
First published
21 Jul 2015

Analyst, 2015,140, 6226-6229

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Single molecular recognition force spectroscopy study of a DNA aptamer with the target epithelial cell adhesion molecule

N. Wang, H. Liu, J. Hao, X. Bai, H. Li, Z. Zhang, H. Wang and J. Tang, Analyst, 2015, 140, 6226 DOI: 10.1039/C5AN00945F

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