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Issue 20, 2015
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Liquid extraction surface analysis field asymmetric waveform ion mobility spectrometry mass spectrometry for the analysis of dried blood spots

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Abstract

Liquid extraction surface analysis (LESA) is a surface sampling technique that allows electrospray mass spectrometry analysis of a wide range of analytes directly from biological substrates. Here, we present LESA mass spectrometry coupled with high field asymmetric waveform ion mobility spectrometry (FAIMS) for the analysis of dried blood spots on filter paper. Incorporation of FAIMS in the workflow enables gas-phase separation of lipid and protein molecular classes, enabling analysis of both haemoglobin and a range of lipids (phosphatidylcholine or phosphatidylethanolamine, and sphingomyelin species) from a single extraction sample. The work has implications for multiplexed clinical assays of multiple analytes.

Graphical abstract: Liquid extraction surface analysis field asymmetric waveform ion mobility spectrometry mass spectrometry for the analysis of dried blood spots

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Publication details

The article was received on 08 May 2015, accepted on 15 Jul 2015 and first published on 15 Jul 2015


Article type: Paper
DOI: 10.1039/C5AN00933B
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Citation: Analyst, 2015,140, 6879-6885
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    Liquid extraction surface analysis field asymmetric waveform ion mobility spectrometry mass spectrometry for the analysis of dried blood spots

    R. L. Griffiths, A. Dexter, A. J. Creese and H. J. Cooper, Analyst, 2015, 140, 6879
    DOI: 10.1039/C5AN00933B

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