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Issue 12, 2015
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Drop-by-drop chemical reaction and sample introduction for capillary electrophoresis

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In this paper, we report a novel sample introduction and chemical reaction strategy by drop-by-drop inkjet injection for an electrophoretically mediated microanalysis (EMMA). This method makes it possible to achieve an on-line introduction of reactant solutions by alternately ejecting small plugs, with an overlapping region of the plugs for mixing the reactants by electrophoresis, supporting chemical reactions, followed by electrophoretic separation of the final compounds. As a proof-of-concept of the method, the EMMA of an inkjetted mixture of 4-fluoro-7-nitrobenzofurazan (NBD-F) and amino acids was carried out as a model chemical reaction. The product NBD-amino acids were quantified by detection with laser induced fluorescence. The optimal conditions for the procedure were: inkjet driving voltage: +40–44 V; pulse width: 20–24 μs; drop-by-drop injection of reactant solutions: alternately 2 drops × 25 times for the amino acid solution and the NBD-F solution; zone overlapping voltage and time: 3 kV and 2 s; incubation time after overlapping: 5 min; separation voltage: 18 kV. Under the optimized conditions, a significant enhancement in sensitivity and a sensitive quantitative analysis were realized. The results obtained were comparable with those using the off-line labeling method. This method is rapid, cost-effective, and readily automated for EMMA.

Graphical abstract: Drop-by-drop chemical reaction and sample introduction for capillary electrophoresis

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Publication details

The article was received on 08 Jan 2015, accepted on 03 Feb 2015 and first published on 05 Feb 2015

Article type: Paper
DOI: 10.1039/C5AN00040H
Citation: Analyst, 2015,140, 3953-3959

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    Drop-by-drop chemical reaction and sample introduction for capillary electrophoresis

    F. Chen, Y. Rang, Y. Weng, L. Lin, H. Zeng, H. Nakajim, J. Lin and K. Uchiyama, Analyst, 2015, 140, 3953
    DOI: 10.1039/C5AN00040H

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