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Issue 8, 2015
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KRAS mutation screening by chip-based DNA hybridization – a further step towards personalized oncology

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Abstract

The use of predictive biomarkers can help to improve therapeutic options for the individual cancer patient. For the treatment of colon cancer patients with anti-EGFR-based drugs, the KRAS mutation status has to be determined to pre-select responders that will benefit from this medication. Amongst others, array-based tests have been established for profiling of the KRAS mutation status. Within this article we describe an on-chip hybridization technique to screen therapeutic relevant KRAS codon 12 mutations. The DNA chip-based platform enables the reliable discrimination of selected mutations by allele-specific hybridization. Here, silver deposits represent robust endpoint signals that allow for a simple naked eye rating. With the here presented assay concept a precise identification of heterozygous and homozygous KRAS mutations, even against a background of up to 95% wild-type DNA, was realizable. The applicability of the test was successfully proven for various cancer cell lines as well as clinical tumour samples. Thus, the chip-based DNA hybridization technique seems to be a promising tool for KRAS mutation analysis to further improve personalized cancer treatment.

Graphical abstract: KRAS mutation screening by chip-based DNA hybridization – a further step towards personalized oncology

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Publication details

The article was received on 12 Nov 2014, accepted on 15 Feb 2015 and first published on 16 Feb 2015


Article type: Paper
DOI: 10.1039/C4AN02086C
Citation: Analyst, 2015,140, 2747-2754
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    KRAS mutation screening by chip-based DNA hybridization – a further step towards personalized oncology

    C. Steinbach, C. Steinbrücker, S. Pollok, K. Walther, J. H. Clement, Y. Chen, I. Petersen, D. Cialla-May, K. Weber and J. Popp, Analyst, 2015, 140, 2747
    DOI: 10.1039/C4AN02086C

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