Chemiluminescence excited paper-based photoelectrochemical competitive immunosensing based on porous ZnO spheres and CdS nanorods

Abstract

A chemiluminescence excited photoelectrochemical (PEC) competitive immunosensor for sensitive and specific detection of the prostate specific antigen (PSA) is firstly developed by combining a microfluidic paper-based device. Firstly, porous ZnO spheres with large surface area and good biocompatibility are attached onto the Au nanoparticle modified paper working electrode, which serve as an effective matrix for antigens. CdS nanorods (NRs) are selected as the photoactive materials due to their excellent fast and long distance electron transport capability, which allow the binding of the horseradish peroxidase-labeled signal antibody onto CdS NRs (CdS NR-Ab-HRP). After a competitive immunoassay format, the CdS NR-Ab-HRP labels are captured onto the electrode surface. The chemiluminescent excitation is produced from the oxidation of luminol by H₂O₂ in the presence of HRP. The more antigens in solution can bind to CdS NR-Ab-HRP the less CdS NR-Ab-HRP can bind to antigens immobilized on the electrode, which result in the decrease of chemiluminescence emission and light absorption, leading to the decrease of photocurrent intensity. The PEC response from CdS NR-Ab-HRP successfully fulfilled the sensitive detection of PSA in the linear range from 0.005 to 150 ng mL⁻¹ with a detection limit of 2.3 pg mL⁻¹. The proposed immunosensor shows excellent analytical performance with high reproducibility and stability, and can become a promising platform for other protein detection.