Issue 10, 2014
From the journal:

Chemical Science

Bioimaging, cellular uptake and dynamics in living cells of a lipophilic fluorescent benzothiadiazole at low temperature (4 °C)†

Alberto A. R. Mota,a   Pedro H. P. R. Carvalho,a   Bruna C. Guido,a   Heibbe C. B. de Oliveira,a   Thereza A. Soares,b   José R. Corrêaa  and  Brenno A. D. Neto*a  

* Corresponding authors

a Laboratory of Medicinal and Technological Chemistry, University of Brasilia (IQ-UnB), Campus Universitario Darcy Ribeiro, CEP 70904970, Brasilia, DF, Brazil
E-mail: brenno.ipi@gmail.com

b Department of Fundamental Chemistry, (dQF-UFPE), s/n – Cidade Universitaria, Av. Jornalista Anibal Fernandes, Recife, PE, Brazil

Abstract

A novel lipophilic fluorescent small-molecule 2,1,3-benzothiadiazole derivative (named here as BTD-AO) was designed, synthesized, characterized and applied as a live cell-imaging probe. Its cellular uptake and cellular dynamics at low temperature (4 °C) using MDA-MB-237 were investigated. Confocal bioimages revealed that the new bioprobe was capable of easily transposing the cell-membrane at low temperatures and also revealed the dynamics of BTD-AO over time. BTD-AO had a high affinity for vesicles (lipid droplets) and had its subcellular location precisely determined during its migration in the cells.

Graphical abstract: Bioimaging, cellular uptake and dynamics in living cells of a lipophilic fluorescent benzothiadiazole at low temperature (4 °C)

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Article information

DOI
https://doi.org/10.1039/C4SC01785D
Article type
Edge Article
Submitted
16 Jun 2014
Accepted
21 Jun 2014
First published
03 Jul 2014

Chem. Sci., 2014,5, 3995-4003

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Bioimaging, cellular uptake and dynamics in living cells of a lipophilic fluorescent benzothiadiazole at low temperature (4 °C)

A. A. R. Mota, P. H. P. R. Carvalho, B. C. Guido, H. C. B. de Oliveira, T. A. Soares, J. R. Corrêa and B. A. D. Neto, Chem. Sci., 2014, 5, 3995 DOI: 10.1039/C4SC01785D

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