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Issue 10, 2014
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Bioimaging, cellular uptake and dynamics in living cells of a lipophilic fluorescent benzothiadiazole at low temperature (4 °C)

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Abstract

A novel lipophilic fluorescent small-molecule 2,1,3-benzothiadiazole derivative (named here as BTD-AO) was designed, synthesized, characterized and applied as a live cell-imaging probe. Its cellular uptake and cellular dynamics at low temperature (4 °C) using MDA-MB-237 were investigated. Confocal bioimages revealed that the new bioprobe was capable of easily transposing the cell-membrane at low temperatures and also revealed the dynamics of BTD-AO over time. BTD-AO had a high affinity for vesicles (lipid droplets) and had its subcellular location precisely determined during its migration in the cells.

Graphical abstract: Bioimaging, cellular uptake and dynamics in living cells of a lipophilic fluorescent benzothiadiazole at low temperature (4 °C)

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Article information


Submitted
16 Jun 2014
Accepted
21 Jun 2014
First published
03 Jul 2014

Chem. Sci., 2014,5, 3995-4003
Article type
Edge Article

Bioimaging, cellular uptake and dynamics in living cells of a lipophilic fluorescent benzothiadiazole at low temperature (4 °C)

A. A. R. Mota, P. H. P. R. Carvalho, B. C. Guido, H. C. B. de Oliveira, T. A. Soares, J. R. Corrêa and B. A. D. Neto, Chem. Sci., 2014, 5, 3995
DOI: 10.1039/C4SC01785D

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