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Issue 7, 2014
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A bacterial DNA quadruplex with exceptional K+ selectivity and unique structural polymorphism

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Abstract

The G-rich sequence d[(G4CT)3G4] was recently identified as a potential quadruplex-forming sequence associated with loci involved in antigenic variation in the human pathogen Treponema pallidum. We found this motif to be enriched in eubacterial genomes. Employing a combination of CD spectroscopy, EPR spectroscopy, analytical ultracentrifugation, and EMSA, we demonstrate that d[(G4CT)3G4] displays unique features among the many G-quadruplex-forming sequences studied so far. To our knowledge d[(G4CT)3G4] shows a so far unprecedented selectivity for K+ with even high concentrations of Na+ unable to induce pronounced G-quadruplex formation. A remarkable continuous and complete transition from an anti-parallel, monomolecular structure into a tetrameric, parallel conformation is observed upon increasing K+-concentrations. Furthermore we investigate the effects of cation selectivity, quadruplex loop composition and length as well as G-tract length on quadruplex conversion.

Graphical abstract: A bacterial DNA quadruplex with exceptional K+ selectivity and unique structural polymorphism

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Publication details

The article was received on 10 Feb 2014, accepted on 20 Mar 2014 and first published on 20 Mar 2014


Article type: Edge Article
DOI: 10.1039/C4SC00440J
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Citation: Chem. Sci., 2014,5, 2809-2818
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    A bacterial DNA quadruplex with exceptional K+ selectivity and unique structural polymorphism

    C. Rehm, I. T. Holder, A. Groß, F. Wojciechowski, M. Urban, M. Sinn, M. Drescher and J. S. Hartig, Chem. Sci., 2014, 5, 2809
    DOI: 10.1039/C4SC00440J

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