A validated over pressured layered chromatography (OPLC) method for separation and quantification of colchicine in Gloriosa superba (L.) tubers from different geographical regions

Abstract

A normal phase on-line OPLC-UV technique was adopted for separation and quantification of colchicine alkaloid in G. superba. The analyzed and developed method was rapid and specific, and selective separation was observed with high peak purity. Validation parameters prove the selectivity and reproducibility of the method and thus can be applied for estimation of a targeted marker in Gloriosa, in various pharmaceutical formulations and also to check phyto-geographical variations among species.