Direct co-culture of endothelial and smooth muscle cells on poly(ε-caprolactone) nanowire surfaces

Abstract

Current cardiovascular implants cause restenosis, calcification and thrombosis due to insufficient integration with native tissue. A thorough understanding of the interaction between the cells and the biomaterial involved is fundamental in a successful implant that promotes healing and tissue regeneration. In this study, we have developed collagen-immobilized nanostructured surfaces from poly(ε-caprolactone) for the growth and maintenance of co-cultures of endothelial and smooth muscle cells. Cell adhesion, morphology and differentiation were investigated using microscopy and western blotting. The results presented here indicate that collagen-immobilized nanostructured surfaces may be good interfaces for use in cardiovascular applications and warrant further investigation.