The first example of a model compound of RNase U2 and its intermediate with CPP directly monitored by ESI-MS

Abstract

A Tb(III) complex of THP [1,4,7,10-tetrakis(2-hydroxypropyl)-cyclen] (THPTb) cleaved RNA phosphate ester bonds at A-specific sites to mimic RNase U2. The intermediates of the adducts of THPTb²⁺ and CPP⁻ (cyclic propylene phosphate) or HPNP⁻ (2-hydroxy propyl-p-nitrophenylphosphate) in the hydrolysis of HPNP were directly monitored by electrospray ionization mass spectrometry (ESI-MS).