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Issue 86, 2014
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Fluorescence detection of natural RNA using rationally designed “clickable” oligonucleotide probes

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Abstract

Herein a reliable approach to the design of effective fluorescent probes for RNA detection is described. The fluorescence signalling of hybridization by internally positioned polyaromatic hydrocarbons and rhodamine dyes was achieved with a low fluorescence background signal, high fluorescence quantum yields at ambient and elevated temperature, high selectivity and signal specificity of the probes when binding to miR-7 and circRNA targets.

Graphical abstract: Fluorescence detection of natural RNA using rationally designed “clickable” oligonucleotide probes

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Publication details

The article was received on 16 Jul 2014, accepted on 10 Sep 2014 and first published on 24 Sep 2014


Article type: Communication
DOI: 10.1039/C4RA07165D
Citation: RSC Adv., 2014,4, 45653-45656
  • Open access: Creative Commons BY license
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    Fluorescence detection of natural RNA using rationally designed “clickable” oligonucleotide probes

    A. Okholm, J. Kjems and K. Astakhova, RSC Adv., 2014, 4, 45653
    DOI: 10.1039/C4RA07165D

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