A novel fluorescent pH probe with valuable pKa based on a twisted intramolecular charge transfer mechanism, and its applications in cell imaging

Fang Yu; Yan Wang; Wencheng Zhu; Yan Huang; Minghui Yang; Hua Ai; Zhiyun Lu

doi:10.1039/C4RA06834C

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A novel fluorescent pH probe with valuable pK_a based on a twisted intramolecular charge transfer mechanism, and its applications in cell imaging†

Fang Yu,‡^a Yan Wang,‡^b Wencheng Zhu,^c Yan Huang,^a Minghui Yang,*^b Hua Ai*^c and Zhiyun Lu*^a

Author affiliations

* Corresponding authors

^a Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, Chengdu, PR China
E-mail: luzhiyun@scu.edu.cn

^b Key Laboratory of Magnetic Resonance and Atomic and Molecular Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Centre for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, PR China
E-mail: yangmh@wipm.ac.cn

^c National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, PR China
E-mail: huaai@scu.edu.cn

Abstract

A novel structurally simple and easily prepared pH probe based on a twisted intramolecular charge transfer signalling mechanism, namely Napa-pp, has been designed and synthesized. In aqueous buffer solution, it has a valuable pK_a of 5.32 that matches the typical pH range of acidic organelles, hence it is applicable for visualizing lysosomes in living cells.

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Article information

DOI: https://doi.org/10.1039/C4RA06834C
Article type: Communication
Submitted: 09 Jul 2014
Accepted: 13 Aug 2014
First published: 20 Aug 2014

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RSC Adv., 2014,4, 36849-36853

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A novel fluorescent pH probe with valuable pK_a based on a twisted intramolecular charge transfer mechanism, and its applications in cell imaging

F. Yu, Y. Wang, W. Zhu, Y. Huang, M. Yang, H. Ai and Z. Lu, RSC Adv., 2014, 4, 36849 DOI: 10.1039/C4RA06834C

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