Issue 83, 2014
From the journal:

RSC Advances

Metabolic variation of HeLa cells migrating on microfabricated cytophilic channels studied by the fluorescence lifetime of NADH†

Gitanjal Deka,‡a   Kazunori Okano,‡*b   Hiroshi Masuhara,c   Yaw-Kuen Lic  and  Fu-Jen Kao*a  

* Corresponding authors

a Institute of Biophotonics, National Yang-Ming University, Linong St. Sec 2, No. 155, Taipei, Taiwan
E-mail: fjkao@ym.edu.tw
Tel: +886-02-28267000 ext. 5713

b Centre for Interdisciplinary Science, National Chiao Tung University, Tin-Ka Ping Photonics Centre, 1001 Ta Hsueh Rd., Hsinchu 30010, Taiwan
E-mail: okano@nctu.edu.tw

c Department of Applied Chemistry and Institute of Molecular Science, National Chiao Tung University, Tin-Ka Ping Photonics Centre, 1001 Ta Hsueh Rd., Hsinchu 30010, Taiwan

Abstract

We report a novel method for studying cellular migration in vitro. Cytophilic microdomains were formed on a cytophobic substrate by laser ablation. HeLa cells were grown on those domains until confluence, and then channels were formed to guide cellular migration. Two-photon excitation fluorescence-lifetime imaging of NADH revealed metabolic variation among migrating and nonmigrating cells.

Graphical abstract: Metabolic variation of HeLa cells migrating on microfabricated cytophilic channels studied by the fluorescence lifetime of NADH

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C4RA06492E
Article type
Communication
Submitted
01 Jul 2014
Accepted
09 Sep 2014
First published
17 Sep 2014

RSC Adv., 2014,4, 44100-44104

Author version available

Download author version (PDF)

Permissions

Request permissions

Metabolic variation of HeLa cells migrating on microfabricated cytophilic channels studied by the fluorescence lifetime of NADH

G. Deka, K. Okano, H. Masuhara, Y. Li and F. Kao, RSC Adv., 2014, 4, 44100 DOI: 10.1039/C4RA06492E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements