Temperature, pH and H-bond synergism for peptide bond formation: synthesis of sequence specific tetra- and penta-peptides without using coupling reagent

Abstract

A reaction temperature of 120 °C, pH of 5.0 and reaction time of 6 h were chosen as the best conditions for the condensation of two nonactivated amino acids to form a peptide bond. Role of H-bond in these reactions was anticipated. The method was validated by procuring sequence specific tetrapeptides and pentapeptides without any disturbance to their chirality.