Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 38, 2014
Previous Article Next Article

Simultaneous determination of a broad range of cardiovascular drugs in plasma with a simple and efficient extraction/clean up procedure and chromatography-mass spectrometry analysis

Author affiliations

Abstract

A simple, easy to use and efficient method was described for simultaneous determination of ten cardiovascular drugs with a broad range of physicochemical properties in rat plasma via online solid phase extraction (online SPE) and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Following a simple centrifugation step, a 10 μL aliquot of the plasma sample was injected directly onto the HPLC system. The LiChrospher® RP-18 ADS (25 mm × 4 mm, 25 μm, Merck) cartridge was washed with 10 mM ammonium acetate buffer (pH 9.5) for 1 min, after which time the analytes were removed by back-flushing directly onto the analytical column (Acclaim 120 C18 column, 150 mm × 4.6 mm, 5 μm) with gradient elution using acetonitrile–10 mM ammonium acetate buffer (pH 3.5) as mobile phase. The flow rate through both columns was 1 mL min−1, and the analytes were quantified using a triple-quadrupole tandem mass spectrometer in multiple-reaction monitoring mode. Linear calibration curves were obtained over the range of 0.2–100 ng mL−1, and the method limits ranged from 0.2 ng mL−1 to 1 ng mL−1 which is sensitive enough for clinical drug monitoring. The intra- and inter-day precisions were in the range of 0.20–2.32%, and the accuracies were between 93.33% and 114.60%. Excellent recoveries from plasma were achieved with a range from 83.52% to 107.38%. The procedure was easier to execute and required less sample handling than methods previously described in the literature. This easy to use and high-throughput method with direct injection of plasma samples for the analysis of multiple cardiovascular drugs may provide a practical solution for tailoring drug dosage in a rational manner to rapidly achieve optimal efficacy and safety of medication.

Graphical abstract: Simultaneous determination of a broad range of cardiovascular drugs in plasma with a simple and efficient extraction/clean up procedure and chromatography-mass spectrometry analysis

Back to tab navigation

Article information


Submitted
06 Feb 2014
Accepted
10 Apr 2014
First published
11 Apr 2014

RSC Adv., 2014,4, 19629-19639
Article type
Paper
Author version available

Simultaneous determination of a broad range of cardiovascular drugs in plasma with a simple and efficient extraction/clean up procedure and chromatography-mass spectrometry analysis

L. Liu, Y. Wen, K. Liu, L. Sun, Y. Lu and Z. Yin, RSC Adv., 2014, 4, 19629
DOI: 10.1039/C4RA01045K

Social activity

Search articles by author

Spotlight

Advertisements