Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins†
We demonstrate high-density labelling of cellular DNA and RNA using click chemistry and perform confocal and super-resolution microscopy. We visualize the crescent and ring-like structure of densely packed RNA in nucleoli. We further demonstrate click chemistry with unnatural amino acids for super-resolution imaging of outer-membrane proteins of E. coli.
- This article is part of the themed collection: Cellular and Tissue Imaging – Luminescent Tags and Probes