A bienzyme-immobilized highly efficient niobium oxide nanorod platform for biomedical application
Abstract
Electrophoretically deposited niobium oxide nanorods have been utilized to fabricate a high performance biosensor for specific cholesterol detection. The orthorhombic structure of the Nb2O5 platform has resulted in the stable and excellent characteristics of the fabricated biosensor. This biosensor has been able to detect cholesterol selectively in a wide detection range [25–500 mg dl−1] with a sensitivity of 0.267 μA/mg dl−1 cm−2 , has a re-usability of 10 times and stability for 6 months, due to the immobilization of the bienzyme onto the matrix, assisted by the intrusion in the Nb2O5 nanorods. The low Michaelis–Menten constant (0.07 mg dl−1) for the biosensor indicates a higher affinity of the bienzyme towards cholesterol. The sensor has also been validated with clinical samples.